Age-dependent failure of axon regeneration in organotypic culture of gerbil auditory midbrain
نویسندگان
چکیده
منابع مشابه
Age-dependent failure of axon regeneration in organotypic culture of gerbil auditory midbrain.
Inferior colliculus (IC) slice cultures from postnatal (P) day 6-8 gerbils exhibit axonal regeneration across a lesion site, and these regrowing processes can form synapses. To determine whether regenerative capacity is lost in older tissue, as occurs in vivo, slices from P12-21-day animals were grown under similar conditions. While these cultures displayed a near complete loss of neurons over ...
متن کاملRegeneration of the auditory midbrain intercommissural projection in organotypic culture.
The aim of this study was to determine whether postnatal mammalian central neurons retain the capacity for axonal regeneration across a lesion site in organotypic cultures of the auditory midbrain. Brain slices from the gerbil inferior colliculus (IC) were obtained from postnatal 6-8 d old animal and cultured for 6-15 d in vitro. IC explants containing an intact commissural projection exhibited...
متن کاملIn vitro analysis of mechanisms underlying age-dependent failure of axon regeneration.
Severed axons of the inferior colliculus (IC) commissure can regenerate across a lesion in organotypic cultures from postnatal day (P) 6 gerbils, but this regenerative capacity is lost by P12 (Hafidi et al. [ 1995] J Neurosci 15:1298-1307, [1999] J Neurobiol 41:267-280). In the present study, we examined the mechanisms underlying this age-dependent failure of axons to regenerate. In P6-P12 hete...
متن کاملAxon regeneration in organotypic slice cultures from the mammalian auditory system is topographic and functional.
In vitro models have frequently been employed to investigate the specificity of the formation of axonal projections during both development and regeneration. Such studies demonstrated pathway, target, and laminar specificity, yet they did not tackle the problem of topography. Here, we addressed the issue of regeneration of spatial specificity at the topographic level by lesioning a precisely or...
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The ability of injured axons to regenerate declines with age, yet the mechanisms that regulate axon regeneration in response to age are not known. Here we show that axon regeneration in aging C. elegans motor neurons is inhibited by the conserved insulin/IGF1 receptor DAF-2. DAF-2's function in regeneration is mediated by intrinsic neuronal activity of the forkhead transcription factor DAF-16/F...
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ژورنال
عنوان ژورنال: Journal of Neurobiology
سال: 1999
ISSN: 0022-3034,1097-4695
DOI: 10.1002/(sici)1097-4695(19991105)41:2<267::aid-neu9>3.0.co;2-d